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Past work on this topic & References

 

There are remarkably few papers on this topic [conductivity used to monitor enzyme catalysis] in the literature (at least that I have been able to find that are relevant to the present work). However it is hardly a recent topic, since Henri used the method to measure gelatin hydrolysis by trypsin in 1903! Conductimetry has been widely applied in micro-biosensors, but I have excluded these (with an exception at the end) as they are  'High-Tech'.

 

For an excellent  general text on enzyme assays I would like to refer you to the book of this title editedby Robert Eisenthal & Mike Danson

(see Google) 

This book contains a chapter written by my PhD supervisor Keith Brocklehurst

 

I also authored a book 'Molecular Enzymology' with Robert who was a very clever & original guy who devised, by lateral thinking, the Direct Linear Plot for kinetic analysis

 

Enzyme catalysed reactions measured by conductivity changes

 

Urease: (this paper is in French)

Hanss, M. & Rey, A. (1971) Biochem et Biophysics Acta, 227, 630-638

 

Cholinesterase: (this paper is in French)

Hanss, M. & Rey, A. (1971) Biochem et Biophysics Acta, 227, 618-629

 

A wide range of enzymes:

Lawrence, A.J. & Moores, G.R. (1972) Eur. J. Biochem. 24, 538-546 

conductimetry in enzyme studies

Trypsin & Chymotrypsin

Hill, G.R. & Tomalin, G. (1982) Anal. Biochem. 120, 165-172

 

Microchip assay for alkaline phosphatase:

Faure, M., Sotta, B. & Gamby, J (2014) Biosensors & Bioelectronics, 58, 61-67

Kechadi,M., Sotta, B. & Gamby, J. (2015) Talanta 132, 785-789

 

These last two references demonstrate the 21st century micro-technology approach to the topic, where reaction volumes are in the low nanolitres and there is no direct electrical contact with the reagents. Fancy kit is needed to fabricate the chips but it could be done on a large scale to give an inexpensive device such as the modern glucose analyser

 

  not to making it look neater - YET!

 

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