My name is Chris Wharton, a retired academic biochemist, now an Emeritus Professor at the University of Birmingham, who worked, during his career, with very hi-tech ( v. expensive!) equipment making measurements of enzyme reaction mechanisms, using primarily spectroscopic, (particularly infrared) & kinetic methods. Latterly I focussed on the mechanisms of antibiotic resistance.
When I retired I decided to try to design and build much more simple apparatus that would be cheap, easy to build, easy to use. It is largely unrelated to what I did during my career but does involve enzymes and kinetics. The kit will hopefully have application in teaching in schools & in universities as well as more generally in the developing world, e.g. for use in clinical testing in remote locations.
I should mention that I have measured several other enzyme reactions (proteases, alkaline phosphatase) using this apparatus and all seem to work well.
I decided to omit the formal vocabulary & theory of classical conductivity as it can act as a barrier to understanding for those who are unfamiliar with it (like me!).
Without doubt the most impressive developments in microsensor systems have been the amperometric blood glucose analysis kits. In my view these are streaks ahead of any other system owing to the pressure of demand. I was able to purchase such a kit for £15 that is fully automated (except the blood taking) & robust for clinical analysis, which is very demanding.
I would like to thank Dr Eva Hyde for help with this project.
The Proverbial 'Cabin or Shed' - where the work is done!